L9894

Sigma

 

Lectin from Dolichos biflorus (horse gram)

agarose conjugate, buffered aqueous suspension

Synonym:Dolichos biflorus agglutinin, DBA

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Description

Biochem/physiol ActionsDBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity,1 and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues.2 Conjugates are prepared from affinity purified lectin.
Physical formSuspension in 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM GalNAc, 0.08% sodium azide

Properties

formbuffered aqueous suspension
extent of labeling2-4 mg protein (Lowry) per mL
matrix 4% beaded agarose
conjugateagarose conjugate
storage temp.2-8°C

Safety

WGK Germany2

References

Cited Reference1. Bird, G. W. G. Blood 21, 366, (1970)
 2. Etzler, M. E., Kabat, E. A. Biochemistry 9, 869, (1970)
referenceEtzler, M. E. Meth. Enzymol. 28, 340, (1972)